Supporting Information: Relevance of the drag force during controlled translocation of a DNA-protein complex through a glass nanocapillary

Quartz capillaries with a 0.3 mm inner diameter and a 0.4 mm outer diameter were purchased from Hilgenberg. They were pulled using the program HEAT = 620, FIL = 0, VEL = 30, DEL = 140, PUL = 150 on a laser-assisted pipette puller (P-2000, Sutter Instruments). A typical pulling time was 0.30 ± 0.03 sec and diameters of the pulled capillaries were in the range of 150−200 nm. All pulled capillaries were shrunken under SEM Merlin (Zeiss) to sizes in the range of 20-120 nm according to Steinbock et al. 1 . Afterwards they were integrated in a PDMS sample cell and attached to a coverslip in a way that cis and trans chambers were connected only via the nanocapillary opening2. The sample cells with nanocapillaries were cleaned with oxygen plasma for 2-5 min at ≈ 50 W and filled with a buffer solution. Different